Nefeli Tsaousi
PhD student
University of the Aegean University Hill, 81100, Mytilene, Lesvos, Greece
WORK EXPERIENCE
01/04/2024 – Current
Research Assistant
IMBRIW - National Monitoring Water NetworkMonitoring and recording the status (quality, quantity, pressures, use) of the country’swaters” (02.5032401/001-006)
Description
- Field sampling.
-Identification of freshwater benthic macroinvertebrates.
-Data entry.
-Application of biomarkers.
-Writing data reports.
Laboratory of Applied Hydrobiology, Agricultural University of Athens
www.zp.aua.gr/hydrobiology-lab/
Address: Iera Odos 75, Athens, 118 55, Athens, Greece
18/09/2022 – 31/10/2023
Research Assistant
Research Project: "Base line study for fisheries management on coastal areas based onlocal ecological knowledge in Amorgos island"
Scientific Director: Stefanos Kalogirou
Funding: Blue Marine Foundation (BMF), Conservation Collective (Cyclades PreservationFund - CPF)
Job description
- Collection, recording and maintenance of biological and abiotic data records (fielddata, biological samples and morphometric data, feeding habits, reproduction data,use of CTD sensors).
-Plankton sampling.
-Periodic inspection of laboratory equipment.
-Data visualisation using R (programming language).
-Writing of deliverables.
-Budget management of the research project (salaries of regular and temporarystaff, subcontracting, procurement of fixed equipment - consumables and travel).
Laboratory of Applied Hydrobiology, Agricultural University of Athens
www.zp.aua.gr/hydrobiology-lab/
Address: Iera Odos 75, Athens, 118 55 , Athens, Greece
[24/09/2021 – 29/09/2022
Research Assistant
Research project: "MOLECULAR IDENTIFICATION OF FISH AUTHENTICITY ANDADULTΕRATION IN GREEK MARKET - FISHmID"
Scientific Director: Eleni Miliou
Funding: European Maritime and Fisheries Fund (EMFF), Fisheries and MaritimeOperational Programme 2014 - 2020,
Website: www.fishmid.aua.gr/
Job Description
-Collection and isolation of DNA samples.
-Design of primers for use in Polymerase Chain Reaction (PCR).
- Design and performance of Polymerase Chain Reaction (PCR), Multiplex PCR, PCRproduct digestion with restriction enzymes (PCR-RFLP).
- Agarose gel electrophoresis (agarose gel electrophoresis).
-Verification of DNA sequences.
-Determination of complete mitochondrial DNA genome.
-Database management (BLAST, CLUSTALW).
-Development of molecular technique-specific protocols for the control ofcommercial adulteration.
-Periodic inspection of laboratory equipment.
-Writing of deliverables.
Laboratory of Applied Hydrobiology, Agricultural University of Athens
www.zp.aua.gr/hydrobiology-lab/
Address: Iera Odos 75, Athens, 118 55, Greece
10/2019 – 08/2021
Internship
Chemical composition analyses in fish tissue and feed:
-Determination of Protein (Internal Method based on AOAC 2001.11,AOAC OfficialMethod 981.10, FOSS Application Note AN 300:FOSS Application Subnote ASN3106:FOSS Application Note AN 3001, METHOD CODE : OE 510-07, Kjeldahl method.- Hellenic Accreditation System (ESYD) - Certified Analyst).
-Determination of total volatile basic nitrogen (TVBN) (Internal Method for distillingan extract deproteinised by perchloric acid based on 95/149/EC and FOSSApplication Note AN 5393, METHOD CODE : OE 510-05).
-Quantification of Fat by Soxhlet method (Internal Method based on protocols991.36, 2003.05 Foss application note AN 301, AN 390,AN 3147, METHOD CODE :OE 510-03 Acid hydrolysis & Soxhlet; - Hellenic Accreditation System (ESYD) -Certified Analyst).
-Quantification of Fat by the Folch method.
-Ash Determination (Internal Method based on AOAC Protocol 938.08, METHODCODE : OE 510-02 - Hellenic Accreditation System (ESYD) - Certified Analyst).
-Moisture determination (Internal Method based on AOAC 950.46, Method Code :OE 510-01 - Hellenic Accreditation System (ESYD) - Certified Analyst).
-Determination of Phosphorus in feed.• Fatty acid esterification.
ECF Farmsystems GmbH
www.ecf-farmsystems.com/
Address: Bessemerstraße 20, 121 03 , Berlin, Germany
07/2019 – 09/2019
Erasmus+ Internship
-Monitoring of physico-chemical water parameters.
-Monitoring and maintenance of water recirculation system (RAS).Other language(s):EnglishLISTENING C2 READING C2 WRITING C2SPOKEN PRODUCTION C2 SPOKEN INTERACTION C2
-Feeding of farmed fish (Tilapia - Oreochromis niloticus).
-Monitoring and maintenance of hydroponics system and cultivation of basil (Basil -Ocimum basilicum).
Laboratory of Applied Hydrobiology, Agricultural University of Athens
www.zp.aua.gr/hydrobiology-lab/
Address: Iera Odos 75, Athens, 118 55, Greece
07/2017 – 08/2017
Internship
Database maintenance and enrichment in systematic botany.
EDUCATION AND TRAINING
02/2026 – Current
PhD Candidate
Department of Marine Sciences, University of the Aegean
City: Mitilini, Lesvos
Country: Greece
Field(s) of study: Marine Ecology
10/2024 – 30/09/2025
Integrated Coastal Management (MSc)
Department of Marine Sciences, University of the Aegean : www.mar.aegean.gr/
City: Mitilini, Lesvos
Country: Greece
01/10/2016 – 21/07/2021
Animal Science (Integrated Master)
Department of Animal Science, Agricultural University of Athens : https://zp.aua.gr/en-gb/homepage-en/
Address: Iera Odos 75, Athens, 118 55, Athens, Greece
Final grade: 7.84
Type ofcredits: ECTS
Number of credits: 300
Thesis: Προσδιορισμός της πλήρουςπρωτοδιάταξης του μιτοχονδριακού DNA του ελληνικού φαγκριού Pagrus pagrus για τηνεκτίμηση της αυθεντικότητάς του.
02/2021 – 06/2021
Minor in Blue Growth and Economy
European University EU - Conexus : www.eu-conexus.eu/en/
Country: Greece
LANGUAGE SKILLS
Mother tongue(s): Greek
Other language(s):English
LISTENING C2 READING C2 WRITING C2SPOKEN PRODUCTION C2 SPOKEN INTERACTION C2
French
LISTENING B2 READING B2 WRITING B2SPOKEN PRODUCTION B2 SPOKEN INTERACTION B2
PUBLICATIONS
2025
Reference: Genes 16, no. 3: 255.
Chatzoglou, Evanthia, Nefeli Tsaousi, Ariadni Spetsieri, Emmanouil E. Malandrakis, andHelen Miliou
2024
Reference: Fishes, 9 (6): 237. MDPI
Tsaousi, N., and Kalogirou, S.
2023
Reference: Genes, 14(6), 1255. MDPI
Chatzoglou, E., Tsaousi, N., Apostolidis, A. P., Exadactylos, A., Sandaltzopoulos, R., Giantsis,I. A., Gkafas, G. A., Malandrakis, E. E., Sarantopoulou, J., Tokamani, M., Triantaphyllidis, G,Miliou, H.
CONFERENCES AND SEMINARS
01/10/2025 – 04/10/2025
12th Symposium of Hellenic Ecological Society (HELECOS), 2025 Athens
Nefeli Tsaousi, Evangelia Smeti, Ioannis Karaouzas, Drosos Koutsoubas, AlexandraMeziti, 2025. Spatial and temporal patterns of biodiversity in estuarine ecosystems ofGreece and investigation of potential trophic relationships - Oral presentation: book ofabstracts p. 171
27/09/2022 – 30/09/2022
Aquaculture Europe 2022 Rimini, Italy
E. Chatzoglou, N. Tsaousi, E. Malandrakis, H. Miliou (2022) THE USE OF RAPIDMOLECULAR TECHNIQUES FOR THE IDENTIFICATION OF E. aeneus AND E. marginatus FROM GREEK CATCHES. - Poster presentation: book of abstracts p. 235
Link: https://eposters.blob.core.windows.net/eas-eposters/AE2022AbstractBook.pdf
04/05/2022 – 06/05/2022
6th International Symposium on Genomics in Aquaculture Granada, Spain
E. Chatzoglou, N. Tsaousi, G. Triantaphyllidis, E. Malandrakis, H. Miliou (2022)MOLECULAR TOOLS FOR RAPID IDENTIFICATION OF SPARIDAE SPECIES IN THE GREEK FISHMARKET. - Poster presentation (P19)
Link: https://doi.org/10.3390/aquacj2020007
04/11/2021 – 06/11/2021
4th International Congress on Αpplied Ichthyology, Oceanography & ΑquaticEnvironment - Hydromedit 2021 : virtual event
E. Chatzoglou, N. Tsaousi, G. Triantaphyllidis, E. Malandrakis, H. Miliou, 2021. The use ofthe mtDNA as a molecular tool towards authenticity of the red porgy Pagrus pagrus. – Oralpresentation
04/10/2021 – 07/10/2021
Aquaculture Europe 2021 Funchal, Madeira
E. Chatzoglou, N. Tsaousi, G. Triantaphyllidis, E. Malandrakis, H. Miliou, 2021. The use ofcomplete mtDNA of the red porgy Pagrus pagrus as a molecular tool towards fishauthenticity. - Oral presentation: book of abstracts p. 235-236
Link: https://eposters.blob.core.windows.net/eas-eposters/AE2021AbstractBook.pdf
04/10/2021 – 07/10/2021
Student workshop: Next-generation aquaculture: the future of EU fish farming,Aquaculture Europe 2021 Funchal, Madeira
Ν. Tsaousi, The complete mitochondrial DNA of the red porgy Pagrus pagrus(Aegean Sea):Species identification through mtDNA comparisons. - Oral presentation (speaker): oralpresentation listing p.26
Link: https://aquaeas.org/_pdf/AE2021BluePages.pdf
01/07/2021
2nd Annual Closing EU-Conexus 2021 : A strategic university partnership for thelong-term : virtual event
N. Tsaousi, The important aspects of the student life within the EU-Conexus alliance. - Oral presentation (speaker)
Link: https://www.eu-conexus.eu/en/events/eu-conexus-2nd-annual-closing-conference-a-strategic-university-partnership-for-the-long-term/
12/04/2020 – 15/04/2020
Aquaculture Europe 2020 : online event
C. Zantioti, E. Chatzoglou, K. Kousouli, N. Tsaoussi, H. Miliou, 2021. Energy and nutrientbalance of Ulva sp. and European sea bass co-culture in RAS under two different diets. - E-poster: Book of abstracts p. 639
07/10/2019 – 10/10/2019
Aquaculture Europe 2019 Berlin, Germany
E. Chatzoglou, M. Karavoulia, C. Zantioti, K. Koussouli, N. Tsaoussi, A. Christofi, P.Kechagia, J. Aubin, H. Miliou, 2019. Integrated cultivation of European sea bassDicentrarchus labrax and Ulva sp. in recirculating aquaculture systems with two differentdiets. - Poster presentation: book of abstracts p. 267
Link: https://www.aquaeas.eu/images/stories/Meetings/AE2019/AE19_Abstracts_FINAL_July_9_web.pdf